Spliced leader RNA of trypanosomes: in vivo mutational analysis reveals extensive and distinct requirements for trans splicing and cap4 formation.
نویسندگان
چکیده
منابع مشابه
Spliced leader trans-splicing
on the forests, wildlife, and indigenous people of this area, WWF warns. It is not necessary to use this area as there is already a total of 2.3 million hectares of idle or derelict land available for palm oil plantations in Kalimantan, an area greater than the proposed plantation, WWF points out. In addition, oil palm is not recommended for planting in areas more than 200 metres above sea leve...
متن کاملTrans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA.
Trypanosoma brucei spliced leader (SL) RNA contains an unusual cap 4 structure consisting of 7-methylguanosine linked to four modified nucleosides. During RNA maturation, trans splicing transfers the first 39 nucleotides of the SL RNA including the cap structure to the 5' end of all mRNAs. Here we show that exposure of permeable trypanosome cells to S-adenosyl-L-homocysteine inhibits methylatio...
متن کاملSpliced leader RNA trans-splicing discovered in copepods.
Copepods are one of the most abundant metazoans in the marine ecosystem, constituting a critical link in aquatic food webs and contributing significantly to the global carbon budget, yet molecular mechanisms of their gene expression are not well understood. Here we report the detection of spliced leader (SL) trans-splicing in calanoid copepods. We have examined nine species of wild-caught copep...
متن کاملSpliced leader RNA-mediated trans-splicing in phylum Rotifera.
In kinetoplastids, Euglena, and four metazoan phyla, trans-splicing has been described as a mechanism for the generation of mature messenger RNAs (mRNAs): 5'-ends of precursor mRNAs are replaced by a short spliced leader (SL) exon from a small SL RNA. Although the full phylogenetic range is unknown, trans-splicing has not been found in vertebrates, insects, plants, or yeast. In animal groups wh...
متن کاملSpliced-leader trans-splicing in freshwater planarians.
trans-Splicing, in which a spliced-leader (SL) RNA is appended to the most 5' exon of independently transcribed pre-mRNAs, has been described in a wide range of eukaryotes, from protozoans to chordates. Here we describe trans-splicing in the freshwater planarian Schmidtea mediterranea, a free-living member of the phylum Platyhelminthes. Analysis of an expressed sequence tag (EST) collection fro...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1996
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1996.tb00811.x